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Cotinine - Nicotine test specifications and instructions

Cotinine Test Specifications

Buy Cotinine Urine Test Here!

One Step Cotinine Test Device

A rapid, one step test for the qualitative detection of Cotinine (nicotine metabolite) in human urine.

For Determination of Smoking Status Only.


Nicotine Test
1. Cassette (CAS-COT urine drug test),
2. width,4mm drug test.
3. Result at 5 minutes
4. cut-off 200ng/mL

Product Overview

The Nicotine Test Cassette (Urine drug test, CAS-COT test) is a lateral flow immunochromatographic assay for the detection of Cotinine in human urine, with cut-off 200ng/mL.


NEGATIVE: Two lines appear.

POSITIVE: One red line appears in the control region (C). No line appears in the test region (T).

INVALID: Control line fails to appear.

The COT One Step Cotinine Test Device (Urine) is a lateral flow chromatographic immunoassay for the detection of Cotinine in human urine at a cut-off concentration of 200 ng/mL. This test will detect other related compounds, please refer to the Analytical Specificity table in this package insert.

This assay provides only a preliminary analytical test result. A more specific alternate chemical method must be used in order to obtain a confirmed analytical result. Gas chromatography and mass spectrometry (GC/MS) is the preferred confirmatory method. Clinical consideration and professional judgment should be applied to any drug of abuse test result, particularly when preliminary positive results are used.


Cotinine is the first-stage metabolite of nicotine, a toxic alkaloid that produces stimulation of the autonomic ganglia and central nervous system when in humans. Nicotine is a drug to which virtually every member of a tobacco-smoking society is exposed whether through direct contact or second-hand inhalation. In addition to tobacco, nicotine is also commercially available as the active ingredient in smoking replacement therapies such as nicotine gum, transdermal patches and nasal sprays. In a 24-hour urine, approximately 5% of a nicotine dose is excreted as unchanged drug with 10% as cotinine and 35% as hydroxycotinine; the concentrations of other metabolites are believed to account for less than 5%.